EVALUATION OF ROUNDUP® TOXICITY IN HUMAN LUNG CELLS

Objective: In this study, toxic effects of Roundup, one the most common glyphosate-based herbicides (GBHs), were assessed on human bronchial epithelial cells (BEAS-2B).
 Material and Method: MTT neutral red uptake assays implemented for evaluation cell viability at 24 48 h. Apoptosis detection was made by Muse analyzer while Hoechst staining employed to detect apoptotic nuclear changes. addition, dichlorofluorescein diacetate assay used assessment reactive oxygen species (ROS) formation.
 Result Discussion: Similar half maximal inhibitory concentrations (IC50) obtained from cytotoxicity assays. Results showed that significant reduction in BEAS-2B started occur 200 µM h 50 at48 treatment times. Roundup treatments both time points found dose-dependently cause apoptosis also observed induce cellular ROS formation. These findings suggest GBHs can stimulate production, as well healthy lung which is important considering inhalation primary exposure routes these group chemicals.